Bio-Rad SmartSpec Plus Spectrophotometer User Manual

Page 38

If there are no standard replicates. SmartSpec Plus will direct you to enter
the concentration of each standard and then to measure the absorbance of
each standard.

•

Use the number keys to input the concentration of standard #1 and press Enter.

You may enter concentrations with as many as eight significant digits, but all
results are reported with four significant digits because SmartSpec Plus
absorbance data are measured to four significant digits.

•

Place the cuvette with standard #1 in SmartSpec Plus and press Read Sample.

SmartSpec Plus will continue to prompt for all subsequent standards until the last
one is read. Before each standard is measured, you will be asked to specify the
concentration of the standard. The units of concentration for all standards must be
the same as those specified before the first standard was read.

•

When collecting data for construction of the standard curve, SmartSpec Plus does
not display the absorbance data, but you may view it at any time by pressing Abs.

•

Press the right arrow key to return to the assay.

Once all the standards are read, SmartSpec Plus will calculate a standard curve. Skip
ahead to C.

ii. If there are standard replicates. If you indicate that one or more of the

standards are read in replicate, then you will be asked if there is the same
number of replicates for each standard.

Same number replicates

for each standard? Yes

Enter concentration of

Standard #2: ________

Insert Standard #2 and

press Read Sample

Enter concentration of

Standard #9: ________

Insert Standard #9 and

press Read Sample

A750 = x.xxx

Standard #1 >=Continue

Insert Standard #1 and

press Read Sample

Enter concentration of

standard #1: ________