Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 7)

4
BioLogic Duo-Flow - - - no method -
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Method
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Run Browser Manual Setup Setup Protocol
wash load 1 2
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Fraction Collector: BioFrac
START
Gradient Pump: F10
Inlet A
1.00
50
50 Inlet B
Flowrate ml/min
%
%
High
limit
0
700
Low
limit psi.
AVR7-3 at port 4
Workstation Valves
SVT5-4 at port 1 SVT3-2 at port 3 SV5-4 at port 2
P L 2 1
2 1
3 4
2 1
3 4
STOP START STOP START STOP
Set
psi.
Econo Gradient Pump 1
EGP %B
1.000
0
0.00 % Split
Flow
Direction
Flowrate ml/min
%
%
Set
Tube number:
ml
Volume left:
Mode: System Local Mode: System Local
Start Tube:
End Tube:
Fraction size:
1
1
20
1.00
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
AVR9-8 at port 6
3 7
8 2
6
5
4
I
ON OFF
Zero Baseline
100% Buffer B
0 2 4 6 8 10
Minutes AU mS/cm
1.50
1.00
0.50
50
0.00
0.0
400.0
300.0
200.0
100.0
Fractions UV Conductivity
Clear
Traces
Resize
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
0.00 ml/min 50 %B
Gradient Pump: F10
WL1 - 280 nm
0.2232 AU
UV
0.15 AU 1.15 AU 0.30 AU
Conductivity
2 psi 0.2583 AU 0.000 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
Settings
File View Utilities Options Window Help
Bio-Rad
Web
Rack: F1 (12-13 mm tubes)
Mode: System Local
QuadTec Detector
Set
Lamp Type
Range
Deuterium
190 - 370 nm
nm 280
Wavelength Selection
nm 260
nm 214
nm 405
Sets fraction collector to system mode
WL2 - 260nm WL3 - 214nm WL4 - 405nm
Fig. 2. Manual Control Screen with QuadTec detector
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