Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 26)

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3.7 pH Electrode Calibration
Prior to starting a run the pH electrode should be calibrated as follows:
a. Remove the electrode from the flow cell and rinse it with deionized water.
b. Place the electrode in pH 7 standard buffer.
c. From the Utilities drop-down menu, select “pH probe calibration”.
d. Enter the temperature and reference pH (at the current temperature) for the
first buffer. Press Set. The temperature can be read from the Display Screen
on the Maximizer.
e. When the pH reading has stabilized, press OK.
f. Rinse the electrode with deionized water.
g. Place the electrode in the pH 10 standard buffer.
h. Enter the reference pH for the second buffer at the current temperature.
Press Set.
i. When the pH reading has stabilized, press OK.
3.8 Manual Screen Chromatogram Window
A feature of the Manual screen is its ability to display up to eight traces of a
chromatogram, including UV/Vis, pH, conductivity, % Buffer B, and pressure
traces over a 10-minute interval. This is useful during column equilibration. The
chromatogram window is displayed at the bottom of the screen, under the valve
control panel (See Figures 7 and 8). Features of the chromatogram window
include:
• The time axis is reset automatically at the end of 10 minutes or may be reset
manually by clicking the Clear Traces button
• The chromatogram window can be enlarged by pressing the Resize button
• A chromatogram trace may be selected for scaling by using the drop-down
menus on the upper right and left of the display
• The Y-axis scale can be changed using the scroll bars on the right or left of the
display
• The maximum and minimum axis settings can be changed by pressing Settings
on the manual screen toolbar
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