Fig. 4. protocol screen – Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
Page 16

13
Isocratic
Flow
Add Step
Linear
Gradient
Load/Inject
Sample
Change
Valve
Hold
Alarm
Zero
Baseline
EGP
Pause
Fraction
Collection
1
2
3
4
5
0.00
0.00
1.00
1.00
Collection Fractions of size 2.00 ml during entire run
Isocratic Flow
1.50
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
100%
0%
Volume: 1.00 ml
Flow: 4.00 ml/min
Linear Gradient
100%
100%
0%
100%
0%
0%
Flow: 4.00 ml/min
6
100% --> 50%
0% --> 50%
7
2.30
Isocratic Flow
15.30
Isocratic Flow
8
18.10
Isocratic Flow
Load/Inject Sample
Zero Baseline
UV Detector
Static Loop
Sample
Auto Inject Valve
Volume: 0.50 ml
Flow: 4.00 ml/min
Volume: 0.80 ml
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
Flow: 4.00 ml/min
Volume: 13.00 ml
Flow: 4.00 ml/min
Volume: 2.80 ml
Flow: 4.00 ml/min
Volume: 8.00 ml
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
A: 25.0 mM Tris pH 8.1
B: 25.0 mM Tris plus 0.5 M NaCI
V
Column
Switching
Repeat
Steps
WL1 - 280nm
0.40
AU
WL2 - 260nm
0.15
AU
WL3 - 214nm
1.15
AU
WL4 - 405nm
0.30
AU
Flow Rate
EGP %B
0 %B
0.00 ml/min
0%
% Split
1.00ml/min
0 %B2
Maximizer + Gradient Pump: F10
UV
Conductivity
438 psi
1.003 AU
1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH
SIM1/SIG
0.548 Volt
7.00 pH
BioLogic DuoFlow -
File
View
Utilities
Options
Edit
Method
New
Method
New
Run Browser
Manual Setup Protocol
wash
load
1
2
Run Notes
Report
PostRun Log
Window
Help
Settings
Cut Copy Paste Delete
Edit
Edit
Bio-Rad
Web
Lamp
26.10
End of Protocol
Fig. 4. Protocol screen
4006208B.qxd 6/28/2004 11:03 AM Page 18
- BioLogic DuoFlow Pathfinder 20 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow Maximizer 80 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow Maximizer 20 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow QuadTec 40 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow QuadTec 10 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow 40 System DuoFlow Chromatography System Starter Kit BioLogic DuoFlow 10 System DuoFlow Chromatography System Starter Kit