Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 3)

II. DuoFlow Maximizer and Pathfinder Systems.....................18
Section 3. System Preparation.......................................................18
3.1 Prime the Workstation Pumps ..................................................20
3.2 Move the AVR7-3 Inject Valve to the Purge Position..................20
3.3 Purge the Workstation Pumps ..................................................21
3.4 Manual Control of the Pumps ...................................................21
3.5 Flush the System Through to the Fraction Collector ..................21
BioFrac Fraction Collection
3.6 Turn on the UV Lamp................................................................22
DuoFlow UV Detector
QuadTec UV/Vis Detector
3.7 pH Electrode Calibration ...........................................................23
3.8 Manual Screen Chromatogram Window ...................................23
3.9 Status Bar ................................................................................24
Section 4. Anion Exchange Separation of Protein Standards ......24
4.1 Overview of the Procedure........................................................24
4.2 Prepare Solutions .....................................................................25
4.3 Prepare Sample ........................................................................26
4.4 Install the UNO Q1 Column.......................................................26
4.5 Prime the Pumps and Equilibrate the UNO Q1 Column.............27
4.6 Create a New Method...............................................................29
Section 5. Ordering Information.....................................................36
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