Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 2)

Table of Contents
Introduction .......................................................................................1
1. Starter Kit Components ..............................................................1
2. Materials You Will Need...............................................................1
I. BioLogic DuoFlow System ....................................................3
Section 1. DuoFlow System Preparation .........................................3
1.1 Prime the Workstation Pumps.....................................................5
1.2 Move the AVR7-3 Inject Valve to the Purge Position ....................5
1.3 Purge the Workstation Pumps ....................................................5
1.4 Manual Control of the Workstation Pumps ..................................6
1.5 Flush the System Through to the Fraction Collector ....................6
BioFrac Fraction Collector
1.6 Turn on the UV Lamp ..................................................................6
DuoFlow UV Detector
QuadTec UV/Vis Detector
1.7 Manual Screen Chromatogram Window......................................7
1.8 Status Bar...................................................................................7
Section 2. Anion Exchange Separation of Protein Standards........8
2.1 Overview of the Procedure..........................................................8
2.2 Prepare Buffers...........................................................................9
2.3 Prepare Sample ..........................................................................9
2.4 Install UNO Q1 Column ............................................................10
2.5 Prime the Pumps and Equilibrate the UNO Q1 Column ............10
2.6 Create a New Method...............................................................10
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