Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 21)

18
II. DuoFlow Maximizer and
Pathfinder Systems
Section 3. System Preparation
When the DuoFlow Maximizer or Pathfinder system is turned on, the Manual
screen is displayed in either Buffer Blending (Figure 7) or Non-Buffer Blending
mode (Figure 8). This screen displays instrument control panels that provide
direct control of the pumps, valves, fraction collector, UV detector, QuadTec
UV/Vis detector, and Econo gradient pump. The arrow in the upper right corner
of the detector control panel toggles between the UV and QuadTec detector
control panels. The button in the upper right hand corner of the valve control
panel toggles between the workstation and Maximizer valve control panels.
BioLogic Duo-Flow - - - no method -
Edit
Method
New
Method
New
Run Browser Manual Setup Setup Protocol
wash load 1 2
Run Notes Report PostRun Log
Advance
Fraction Collector: BioFrac
START
Maximizer+Gradient Pump: F10
pH
Tris (25 mM)
1.00
8.10
0 Inlet B
Flowrate ml/min
%
High
limit
0
700
Low
limit psi.
AVR7-3 at port 4
Workstation Valves
SVT5-4 at port 1 SVT3-2 at port 3 SV5-4 at port 2
P L 2 1
2 1
3 4
2 1
3 4
STOP START STOP START STOP
Set
Event Mark
OFF ON
psi.
Econo Gradient Pump 1
EGP %B
1.000
0
0.00 % Split
Flow
Direction
Flowrate ml/min
%
%
Set
Tube number:
ml
Volume left:
Mode: System Local Mode: System Local Mode: System Local
Start Tube:
End Tube:
Fraction size:
1
1
20
1.00
1.0
I
AVR9-8 at port 5
3 7
8 2
6
5
4
I
AVR9-8 at port 6
3 7
8 2
6
5
4
I
UV Detector
Chart Recorder
Signal Import Module
SIM 1 is connected
ON OFF
Zero Baseline
100% Buffer B
0 2 4 6 8 10
Minutes AU mS/cm
1.50
1.00
0.50
50
-0 0.00 0.0
400.0
300.0
200.0
100.0
Fractions UV Conductivity
Clear
Traces
Resize
UV range (AU):
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
0.00 ml/min 0 %B2
Gradient Pump: F10 UV Conductivity
1 psi 0.2577 AU 3.63 mS/cm
QuadTec
WL1 - 280 nm
0.2225 AU
Econo Gradient
Pump
SIM1/pH SIM1/SIG
Settings
File View Utilities Options Window Help
Bio-Rad
Web
Rack: F1 (12-13 mm tubes)
Setup
Switch to the QuadTec faceplate
0.000 Volt 6.53 pH
Fig. 7. Manual screen (Buffer Blending mode)
4006208B.qxd 6/28/2004 11:03 AM Page 23