Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 34)

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b. Click on the Fraction Collector button in the Available Devices box. A dialog
box will appear asking you to choose the type of collector; i.e., a generic
collector, a Model 2110, or BioFrac. Click on BioFrac and click the OK
button. You will now see the BioFrac fraction collector in the Devices in
Setup box. The F1 Rack (12–13 mm tubes) is automatically selected.
c. If you are using a QuadTec UV/Vis detector, click on the Detectors button in
the Available Devices box. A dialog box will appear asking you to choose a
detector. Select QuadTec and check each of the four wavelengths boxes.
Enter the wavelengths: (1) 280 nm, (2) 260 nm, (3) 214 nm, and (4) 405 nm.
Press OK.
d. The Setup is now complete. To save the device setup, choose Save Setup
under the File menu and enter a name for your Setup.
e. You are now ready to program the separation steps for your method. To
program your method, press the Protocol icon on the tool bar.
Isocratic
Flow
Add Step
Linear
Gradient
Load/Inject
Sample
Change
Valve
Hold
Alarm
Zero
Baseline
EGP
Pause
Fraction
Collection
1
2
3
4
5
0.00
0.00
1.00
1.00
Collection Fractions of size 2.00 ml during entire run
Volume Description Parameters
Isocratic Flow
1.50
pH 8.10 Volume: 1.00 ml
Flow: 4.00 ml/min
Linear Gradient
0%B
pH 8.10
0%B
pH 8.10
0%B -> 25%B5
pH 8.10
50%B
pH 8.10
0%B
6
7
2.30
Isocratic Flow
15.30 Isocratic Flow
8 18.10 Isocratic Flow
Load/Inject Sample
Zero Baseline UV Detector
Static Loop
Sample
Auto Inject Valve Flow: 4.00 ml/min
Volume: 0.50 ml
Flow: 4.00 ml/min
Volume: 0.80 ml
Flow: 4.00 ml/min
Volume: 13.00 ml
Flow: 4.00 ml/min
Volume: 2.80 ml
Flow: 4.00 ml/min
Volume: 8.00 ml
V
Column
Switching
Repeat
Steps
WL1 - 280nm
0.40 AU
WL2 - 260nm
0.15 AU
WL3 - 214nm
1.13 AU
WL4 - 405nm
0.30 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
BioLogic DuoFlow - - - -
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash load 1 2
Run Notes Report PostRun Log
Window Help
Settings Cut Copy Paste Delete Edit
Edit
Bio-Rad
Web
Lamp
26.10 End of Protocol
Fig. 10. Protocol editor
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