Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 14)

11
Program the Instrument Setup
In the Setup screen select the instruments and devices to be used for the Starter
Kit method. The icons grouped on the left side of the screen (refer to Figure 3,
Available Devices) show all the instruments and devices that can be connected
to the BioLogic DuoFlow systems.
The list of devices in the right box (Devices in Setup) identifies those devices
selected for use with a specific method. The initial default Devices in Setup are a
UV detector, conductivity monitor, and an AVR7-3 inject valve. These come
Devices in setup
UV Detector
Signal Import Module 2 pH Range: 0.00 to 14.00 pH
BioFrac Fraction Collector, Rack: F1 (12-13 mm tubes)
SV5-4 Valve - Inlet A
SV5-4 Valve - Inlet B
Conductivity Monitor
AVR7-3 Valve - Sample Inject Port 4
Available Devices
Gradient Pump: F10
SVT3-2
Valve
AVR7-3
Valve
SV5-4
Valve
AVR9-8
Valve
Detectors
Fraction
Collector
SV3
INJECT
P
U
M
P
Aux Load
Pump
Inlet A:
Inlet B:
BioLogic Duo-Flow - - - -
Edit
Method
New
Method
New
Run Browser Manual Setup Delete Protocol
wash
load
1
2
Run Notes Report PostRun Log Settings
Port 1
Port 2
WL1 - 280nm
0.40 AU
WL2 - 260nm
0.15 AU
WL3 - 214nm
1.15 AU
WL4 - 405nm
0.30 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH
File View Utilities Options Window Help Edit
Bio-Rad
Web
Buffer Blender
Inlet A is assigned to SV5-4 Valve - at Port 1
Inlet B is assigned to SV5-4 Valve - at Port 2
25 mM Tris-HCl, pH 8.1
25 mM Tris-HCl, pH 8.1 plus 0.5M NaCl
Fig. 3. Setup editor
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