Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 20)

17
Advance
Frac. Collector
Divert Valve
1 3 2 4 5 6 7 8 9 10 11 12 13 14
100.0% Buffer B
UV Detector
Set
UV Range
Zero
Baseline
Event
mark
Protocol: > 1 0.00 Collection Fractions of size 2.00 ml during entire run
00:00:00 00:02:00 00:04:00 00:06:00
Hr:Min:Sec AU mS/cm
Collect
Waste
Grad. Pump
Chart Recorder
700
High psi
0
Low psi
QuadTec
Zero
Baseline
1.0
0.0
40.0
50.0
30.0
20.0
10.0
-10.0
Fractions
Conductivity QuadTec (280 nm)
Full View
0.100
0.075
0.050
-50.0
0.0
0.025
-0.000
BioLogic Duo-Flow - Demo Chromatography - Standard UV Detector - Sample Run
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol
wash
load
1
2
Run Notes Report PostRun Log
Window Help
Settings
WL1 - 280nm
0.2223 AU
Flow Rate EGP %B
0 %B 0.00 ml/min 0%
% Split
Run Time
0:00.0
Run Volume
0.0 ml
Step Time Left Fraction Vol. Left Valve Info
0.00ml/min 50 %B
Maximizer + Gradient Pump: F10
0.15 AU 1.15 AU 0.30 AU
Conductivity
0 psi 0.2580 AU 3.63 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.000 Volt 8.05 pH
Edit
Bio-Rad
Web
WL2 - 260nm WL3 - 214nm WL4 - 405nm
Fig. 6. Run screen QuadTec UV/Vis traces
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