Bio-Rad Trans-Blot® Plus Cell User Manual

Page 9

Fig. 2.

4. If necessary, adjust the position of the cathode leads (black) on the lid so that

they correspond to the position of the cathode electrode plate (black) within the
tank.

5. Position the Trans-Blot Plus cell on a magnetic stir plate.

6. Add transfer buffer to the appropriate fill line. Choose the fill line that is

appropriate for the number of gel holder cassettes that you are using.

Note: In order to avoid overflow and electrical hazards, do not fill the tank beyond
the indicated buffer level lines marked on the tank.

7. Place the stir bar in the tank.

8. Start cooling. Cooling is required for high intensity field conditions and is

recommended for prolonged, unsupervised runs. Place the Super cooling coil
into its designated slot at the rear face of the tank. Connect the Super cooling
coil to the refrigerated circulator according to the manufacturer's instructions.
Avoid restrictive fittings, internal diameter reductions and excessive extensions
of tubing. Turn on power to the stir plate.

Note: To test that the cooling system is functioning properly before the first transfer
run is initiated, place the Trans-Blot Plus cell on the magnetic stirrer and pour in
11 L of buffer (or water). With no power applied to the tank, the buffer should cool
by 5°C in approximately 20 min.

Cathode Plate (Black)

Super Cooling Coil

One Gel Holder Cassette Loaded

Two Gel Holder Cassettes Loaded

Three Gel Holder Cassettes Loaded

Anode Plate (Red)

Gel Holder Cassette (1)

Anode Plate (Red)

Gel Holder Cassettes (2)

Cathode Plate (Black)

Super Cooling Coil

Anode Plate (Red)

Gel Holder Cassettes (3)

Cathode Plate (Black)

Super Cooling Coil

SLOT

Trans-Blot Plus Cell

MAX LEVEL

MIN LEVEL

1 CASSETTE

Anode Plate (Red)

Gel Holder Cassettes (2)