Bio-Rad Trans-Blot® Plus Cell - Manual (Page 14)

Table 3.1 SDS-PAGE Gels
These conditions were determined empirically using 12.5% Tris-HCl Criterion gels
and prestained SDS-PAGE molecular weight standards (Catalog # 161-0318). See
Section 3.3 for buffer formulations.
High Intensity Field Conditions (Cooling Required)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
Towbin 60V/2A* 45 min 100V/2A 45 min 100V/1.5A 60 min
90V/3A 15–30 min 120V/2.4A 15–30 min 130V/~2.3A 15–30 min
CAPS 41V/2A 15 min 66V/2A 30 min 95V/2A 30 min
60V/3A 15 min 100V/3A 15–30 min 120V/2.5A 15–30 min
Carbonate 25V/2A 15 min 40V/2A 30 min 55V/2A 30 min
35V/3A 15 min 55V/3A 15–30 min 80V/3A 15–30 min
Standard Field Conditions (Cooling Recommended)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
Towbin 10V/0.3A 16 Hrs. 10V/0.2A 16 Hrs. 10V/0.15A 16 Hrs.
20V/ 0.7A 20V/0.4A 20V/0.3A
30V/ 1.1A 30V/0.7 A 30V/0.5 A
CAPS 10V/ 0.35A 4 Hrs. 10V/0.2A 4 Hrs. 10V/0.2A 4 Hrs.
20V/0.8A 20V/0.55 A 20V/0.4A
30V/0.9A 30V/0.6A
Carbonate 10V/0.7A 4 Hrs 10V/0.4A 4 Hrs 10V/0.33A 4 Hrs
20V/1A 20V/0.7A
30V/1A
* When running under constant voltage, the PowerPac 200 power supply will automatically cross over to constant
current if the power supply's current limit is reached.
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