Bio-Rad Trans-Blot® Plus Cell - Manual (Page 8)

2.2 Additional Components
Magnetic stir plate
A magnetic stir plate with a surface area that is sufficient to accommodate the
Trans-Blot Plus cell is required for constant stirring of transfer buffer during
electrophoresis. Recommended stir plates include the PC-610 from Corning, the
Cimarec 3 from Thermolyne and the Vel A from Cole Parmer.
Refrigerated circulator
The Trans-Blot Plus cell requires a refrigerated circulator to work with the Super
cooling coil for optimal results. The recommended minimum cooling capacity of the
refrigerated circulator is 300W at 20°C and minimum pump flow rate is 4 L/min.
Recommended chillers include the Model RTE-7 from Thermo NESLAB and the
Model WKL 26 from Thermo Haake.
Tubing
Tubing with a 3/8 " internal diameter is required to connect both ends of the Super
cooling coil to the refrigerated circulator and for draining the tank using the
quick-release fitting and drain port.
Gel/ Cassette Assembly Tray
The optional gel/ cassette assembly tray is large enough to accommodate a gel
holder cassette and the buffer required for sandwich assembly and may be
purchased separately (see Product Information).
2.3 Setting up the tank and cooling system
1. Prepare the transfer buffer. See Section 3.3 for buffer formulations. Generally,
15 liters of transfer buffer will suffice for electrophoresis, gel equilibration, and
sandwich assembly.
2. Position the anode (red) electrode card into the tank, in the slot that is nearest
the front face of the tank (see Figures 1 and 2). This is the only position
possible for the anode plate since it is the only position that will provide a
connection between the plate electrode and the anode leads in the lid.
Tighten the electrode card in place with the white nylon screw.
3. Position the cathode (black) electrode card into the third, fourth or fifth slot from
the front (4 cm, 7 cm, or 10 cm away from the anode card), depending on
whether you will be transferring one, two, or three gel-containing cassettes,
respectively (see Figure 2). Tighten the electrode card in place with the white
nylon screw.
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