Bio-Rad Trans-Blot® Plus Cell User Manual

Page 2

Table of Contents

Page

Section 1 General Information .........................................................................1

1.1 Introduction....................................................................................................1
1.2 Specifications ................................................................................................2
1.3 Safety.............................................................................................................3

Section 2 Set Up and Basic Operation of the Trans-Blot Plus Cell .............5

2.1 Components ..................................................................................................5
2.2 Additional

Components .................................................................................6

2.3

Setting up the Tank and Cooling System .....................................................6

2.4

Assembling the Gel Sandwich and Cassette................................................8

2.5 Transfer .........................................................................................................9
2.6

Draining the Tank ........................................................................................10

2.7

Running Acidic Transfers ............................................................................10

Section 3 Transfer Conditions .......................................................................11

3.1

General Guidelines and Running Conditions .............................................11

3.2

Notes on Electrophoretic Transfer Conditions ............................................15

3.3 Buffer

Formulations .....................................................................................17

Section 4 Strategies for Optimizing Electrophoretic Transfer ...................21

4.1

Optimizing Protein Transfer ........................................................................21

4.2

Optmizing DNA and RNA Transfer .............................................................23

Section 5 Choice of Blotting Membranes.....................................................23

5.1

Protein Blotting Membranes........................................................................23

5.2

DNA and RNA Blotting Membranes............................................................24

Section 6 Troubleshooting .............................................................................25

Section 7 Maintenance....................................................................................28

Section 8 Product Information .......................................................................29

Section 9 References ......................................................................................30

Section 10 Warranty ..........................................................................................31