Bio-Rad Trans-Blot® Plus Cell User Manual
Page 2

Table of Contents
Page
Section 1 General Information .........................................................................1
1.1 Introduction....................................................................................................1
1.2 Specifications ................................................................................................2
1.3 Safety.............................................................................................................3
Section 2 Set Up and Basic Operation of the Trans-Blot Plus Cell .............5
2.1 Components ..................................................................................................5
2.2 Additional
Components .................................................................................6
2.3
Setting up the Tank and Cooling System .....................................................6
2.4
Assembling the Gel Sandwich and Cassette................................................8
2.5 Transfer .........................................................................................................9
2.6
Draining the Tank ........................................................................................10
2.7
Running Acidic Transfers ............................................................................10
Section 3 Transfer Conditions .......................................................................11
3.1
General Guidelines and Running Conditions .............................................11
3.2
Notes on Electrophoretic Transfer Conditions ............................................15
3.3 Buffer
Formulations .....................................................................................17
Section 4 Strategies for Optimizing Electrophoretic Transfer ...................21
4.1
Optimizing Protein Transfer ........................................................................21
4.2
Optmizing DNA and RNA Transfer .............................................................23
Section 5 Choice of Blotting Membranes.....................................................23
5.1
Protein Blotting Membranes........................................................................23
5.2
DNA and RNA Blotting Membranes............................................................24
Section 6 Troubleshooting .............................................................................25
Section 7 Maintenance....................................................................................28
Section 8 Product Information .......................................................................29
Section 9 References ......................................................................................30
Section 10 Warranty ..........................................................................................31
Copyright© (2002) Bio-Rad Laboratories, Inc. All rights reserved.