Bio-Rad Trans-Blot® Plus Cell - Manual (Page 16)

Table 3.3 Isoelectric Focusing, Native, Acid Urea Gels, Basic
Proteins
These conditions were determined empirically using 12.5% Tris-HCl Criterion
gels and native horse myoglobin samples. Read about acidic transfers in Section 2.7
of this manual.
High Intensity Field Conditions (Cooling Required)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
0.7% Acetic 50V/2A 45 min 70V/2A 60 min 100V/1.9A 60 min
acid , pH 2.8 70V/3A 15–30 min 110V/3A 15–30 min 125V/2.4A 15–30 min
Standard Field Conditions (Cooling Recommended)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
0.7% Acetic 10V/0.4A 16 Hrs. 10V/0.25A 16 Hrs. 10V/0.15A 16 Hrs.
acid , pH 2.8 20V/0.8A 20V/0.55A 20V/0.35A
30V/0.8A 30V/0.55A
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