Bio-Rad Trans-Blot® Plus Cell User Manual

Page 10

2.4 Assembling the Gel Sandwich and Cassette Assembly

Each gel sandwich will contain the gel and membrane sandwiched between

two pieces of blot absorbent filter paper.

To prevent contamination, always wear gloves when handling the gels,

membranes and filter paper to prevent contamination.

An optional gel/ cassette assembly tray is available for the Trans-Blot Plus cell

(see Product Information). This tray is large enough to accommodate the gel holder
cassette during sandwich assembly. The lid of the tray can be used for soaking
membranes.

Fig. 3.

1. For each gel, cut one piece of membrane and two pieces of filter paper to the

dimensions of the gel. Pre-cut membranes and filter papers are available (see
Product Information).

2. Equilibrate gels and membranes by soaking them in transfer buffer for 15 minutes.

3. Pour ~ 3 liters of transfer buffer into a tray for assembly of the cassettes.

4. Place the black cassette plate into the tray with the clamps in their fully extended

position.

5. Place a fiber pad on the black cassette plate, making sure it is thoroughly wet.

6. Place a piece of filter paper on top of the fiber pad. Make sure there is enough

buffer to thoroughly wet the filter paper.

7. Carefully place the equilibrated gel on top of the filter paper.

Gel Holder Cassette

(Red Plate)

Hinge

Fiber Pad

Filter Paper

Gel

Membrane

Gel Holder Cassette
(Black Plate)

Clamp

Fiber Pad