Bio-Rad Trans-Blot® Plus Cell - Manual (Page 15)

Table 3.2 Native Gels
These conditions were determined empirically using 12.5% Tris-HCl Criterion
gels and native horse myoglobin samples. See Section 3.3 for buffer formulations.
High Intensity Field Conditions (Cooling Required)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
Towbin 50V/2A 45 min 90V/2A 45 min 100V/1.7A 60 min
(no methanol) 80V/3A 15–30 min 100V/2.2A 15–30 min 130V/2.3A 15–30 min
Standard Field Conditions (Cooling Recommended)
PLATE ELECTRODE DISTANCE
4 cm 7 cm 10 cm
Buffer Power Run Power Run Power Run
conditions Time conditions Time conditions Time
Towbin 10V/0.3A 16 Hrs. 10V/0.2A 16 Hrs. 10V/0.15A 16 Hrs.
(no methanol) 20V/0.7A 20V/0.4A 20V/0.3A
30V/1.1A 30V/0.7A 30V/0.5A
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