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Caps buffer – Bio-Rad Trans-Blot® Plus Cell User Manual

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CAPS Buffer

CAPS-based transfer buffers (10 mM CAPS, 10% methanol, pH 11) may be

preferable for transfers of high molecular weight proteins (e.g. >50 000 Da) and in
cases where the glycine component of Towbin buffer may interfere with downstream
protein sequencing applications.

CAPS Buffer with 20% Methanol

10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
20% methanol, pH 11

a) Using dry reagents:

2.21 g CAPS

600 ml deionized water (dd H

2

O)

Adjust to pH 11 with NaOH

add dd H

2

O to 800 ml

200 ml methanol

CAPS Buffer with 10% Methanol

10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
10% methanol, pH 11

a) Using dry reagents:

2.21 g CAPS

600 ml deionized water (dd H

2

O)

Adjust to pH 11 with NaOH

add dd H

2

O to 900 ml

100 ml methanol

CAPS Buffer with 15% Ethanol

10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid),
15% ethanol, pH 11

a) Using dry reagents:

2.21 g CAPS

600 ml deionized water (dd H

2

O)

Adjust to pH 11 with NaOH

add dd H

2

O to 850 ml

150 ml ethanol

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