5 storage conditions, 6 important notes – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual
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1.5 Storage Conditions
Table 1.2. Storage conditions for Mini-PROTEAN precast gels. Store gels flat. Shelf life is from date of manufacture;
expiration dates are printed on the packaging.
Storage
Temperature
Gel Formulation
Shelf Life
2–8°C
Mini-PROTEAN TGX
12 months
Mini-PROTEAN TGX Stain-Free
12 months
Mini-PROTEAN Tris-Tricine
12 weeks
Mini-PROTEAN TBE
12 weeks
Mini-PROTEAN TBE-urea
8 weeks
1.6 Important Notes
Use each Mini-PROTEAN precast gel as soon as possible after removing it from the storage pouch.
Improper storage of Mini-PROTEAN precast gels can produce artifacts. Store gels flat and at 2–8°C.
Avoid freezing or prolonged storage above 8°C. If your gels have been stored improperly, discard them.
Do not run more than one gel type in the same apparatus at the same time. Different gel percentages
and formulations have different conductivities and different run times.
With the Mini-PROTEAN Tetra cell:
n
When running 1–2 gels:
Use the electrode assembly (with banana plugs), not the companion running module
(without banana plugs)
Do not place the companion running module in the tank. Doing so generates
excessive heat and degrades the quality of the electrophoretic separation
n
When running 3–4 gels, use both the electrode assembly and companion running module
n
When using voltages >200 V, fill the outer buffer chamber to the 4 gel (800 ml) mark
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