Appendix b: buffers, Buffers, Running buffers – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual
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Buffers
B
Running Buffers
10x SDS-PAGE (1 L)
250 mM Tris, 1 .92 M glycine, 1% SDS, pH 8 .3
(catalog #161-0732)
Tris base
30.3 g
Glycine
144.1 g
SDS
10 g
diH
2
O
to 1 L
Do not adjust the pH (~pH 8.3)
10x Native PAGE (1 L)
250 mM Tris, 1 .92 M glycine, pH 8 .3
(catalog #161-0734)
Tris base
30.3 g
Glycine
144.1 g
diH
2
O
to 1 L
Do not adjust the pH (~pH 8.3)
10x Tris-Tricine (1 L)
1 M Tris, 1 M Tricine, 1% SDS, pH 8 .3
(catalog #161-0744)
Tris base
121.1 g
Tricine
179.2 g
SDS
10 g
diH
2
O
to 1 L
Do not adjust the pH (~pH 8.3)
10x TBE (1 L)
890 mM Tris, 890 mM boric acid, 20 mM EDTA
(catalog #161-0741)
Tris base
107.8 g
Boric acid
55.0 g
EDTA
5.8 g
diH
2
O
to 1 L
Do not adjust the pH (~pH 8.3)
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