1 introduction, 2 mini-protean tbe-urea gels, 1 gel composition – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual
Page 26: 2 gel selection guide, Denaturing nucleic acid page
Migration charts for DNA
standards on Mini-PROTEAN
TBE-urea gels.
20
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Denaturing Nucleic
Acid PAGE
8
8.1 Introduction
Mini-PROTEAN
®
TBE-urea gels are used for separation of small RNA and single-stranded DNA (ssDNA)
fragments. Applications include oligonucleotide analysis, RNase protection assays, and northern
blotting.
8.2 Mini-PROTEAN TBE-Urea Gels
8.2.1 Gel Composition
Gel buffer
89 mM Tris, 89 mM boric acid, 2 mM EDTA, 7 M urea, pH 8.3
Crosslinker
3.3% C
Stacking gel
4% T, 3.3% C
Storage buffer
89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3, NaN
3
Shelf life
8 weeks at 2–8°C; expiration date is printed on the packaging
8.2.2 Gel Selection Guide
Gel Percentage
Optimum Separation Range
10%
25–300 nt
15%
10–50 nt