Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 13)

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the short plates sit just below the notch at the top of
the green gasket (C).
If running more than 2 gels, repeat steps 2–6 with the
companion running module.
7. Place the electrophoresis module into the tank (F) and
fill the buffer chambers with 1x running buffer:
n
200 ml in the inner buffer chamber
n
550 ml (1–2 gels) or 800 ml (3–4 gels, or
>200 V) in the outer buffer chamber
8. Wash the sample wells with running buffer (if this was
not done earlier).
9. Load samples and run the gels using the running
conditions appropriate to your application. Stop the run when the dye front reaches the reference
line imprinted on the bottoms of the cassettes.
2.4 Removing the Gel
1. After electrophoresis is complete, turn off the power supply and disconnect the electrical leads.
2. Remove the lid from the tank and remove the gels from the cell. Pour off and discard the running
buffer.
3. To open the cassette, align the arrow on the opening lever with the arrows marked on the cassette
and insert the lever between the cassette plates at indicated locations. Apply downward pressure to
break each seal. Do not twist the lever.
4. Pull the two plates apart from the top of the cassette, and gently remove the gel.
F
Instruction Manual and Application Guide