Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 21)

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5.2 Stain-Free Workflow
Analyze the Separation
Perform Electrophoresis
SDS-PAGE (Chapter 3)
Native PAGE (Chapter 4)
2-D Electrophoresis (Chapter 9)
Stain the Gels for Total Protein
(Chapter 10)
Blot the Gels
(Chapter 11)
Activate/Image Gels
(Chapter 5)
5.3 Electrophoresis with Mini-PROTEAN TGX Stain-Free Gels
Mini-PROTEAN TGX Stain-Free gels are made and packaged without SDS, so they can be used for
both SDS and native PAGE applications. To perform electrophoresis with these gels, prepare the
sample and running buffers, set up the Mini-PROTEAN Tetra cell, and perform the run as directed in
Chapters 2–4.
Use unstained standards with Mini-PROTEAN TGX Stain-Free gels, as some prestained
standards are not compatible with stain-free technology. To monitor electrophoresis, use
10 µl of a 1:1 mixture of Precision Plus Protein
™ unstained (catalog #161-0363) and
Precision Plus Protein All Blue protein standards (catalog #161-0373).
5.4 Stain-Free Detection
Image Mini-PROTEAN TGX Stain-Free gels and blots in a compatible imager. The imager activates the
reaction between the proteins and trihalo compounds in the gel to enable visualization.
n
Immediately place the gel in the tray of the imager; no fixation or rinsing steps are required.
Prolonged rinsing may diminish image quality and lead to gel deformation
n
If desired, stain the gel with any TGX-compatible stains after imaging. Certain stains, if used
prior to imaging, eliminate detection capability
Refer to the Gel Doc EZ Stain-Free Sample Tray Instruction Manual (bulletin 10019634) or the ChemiDoc MP
System with Image Lab Software Instruction Manual (bulletin 10022469) for detailed instructions.
Instruction Manual and Application Guide