Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 24)

Nondenaturing Nucleic
Acid PAGE
7
7.1 Introduction
Mini-PROTEAN ® TBE gels are used to separate small double-stranded DNA (dsDNA) fragments,
particularly PCR products. DNA molecules have nearly uniform mass-to-charge ratios, allowing
nondenaturing nucleic acid PAGE to separate dsDNA by mass using a continuous TBE buffer system.
7.2 Mini-PROTEAN TBE Gels
7.2.1 Gel Composition
Gel buffer
89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3
Crosslinker 3.3% C
Stacking gel 4% T, 3.3% C
Storage buffer 89 mM Tris, 89 mM boric acid, 2 mM EDTA, NaN
3
Shelf life 12 weeks at 2–8°C; expiration date is printed on the packaging
7.2.2 Gel Selection Guide
Gel Percentage Optimum Separation Range
5% 200–2,000 bp
10% 50–1,500 bp
15% 20–1,000 bp
4–20% 10–2,000 bp
Migration charts for DNA
standards on Mini-PROTEAN
TBE gels.
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