Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 44)

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Sample Buffers
2x SDS-PAGE (Laemmli, 30 ml) 62 .5 mM Tris-HCl, pH 6 .8, 2% SDS, 25% glycerol, 0 .01%
(catalog #161-0737) bromophenol blue, 5% β-mercaptoethanol (added fresh)
0.5 M Tris-HCl, pH 6.8 3.75 ml
50% Glycerol 15.0 ml
1.0% Bromophenol blue 0.3 ml
10% SDS 6.0 ml
diH
2
O to 30 ml
Add β-mercaptoethanol (50 µl to 950 µl sample buffer)
before use.
2x Native PAGE (30 ml) 62 .5 mM Tris-HCl, pH 6 .8, 40% glycerol, 0 .01%
(catalog #161-0738) bromophenol blue
0.5 M Tris-HCl, pH 6.8 3.75 ml
50% Glycerol 24 ml
1.0% Bromophenol blue 0.3 ml
diH
2
O to 30 ml
2x Tricine (30 ml) 200 mM Tris-HCl, pH 6 .8, 2% SDS, 40% glycerol, 0 .04%
(catalog #161-0739) Coomassie Brilliant Blue G-250, 2% β-mercaptoethanol
(added fresh)
1.0 M Tris-HCl, pH 6.8 6.0 ml
100% Glycerol 12.0 ml
10% SDS 6.0 ml
Coomassie Blue G-250 12.0 mg
diH
2
O to 30 ml
Add β-mercaptoethanol (20 µl to 980 µl sample buffer)
before use.
5x Nucleic acid (10 ml) 50 mM Tris-HCl, pH 8 .0, 5 mM EDTA, 25% glycerol, 0 .2%
(catalog #161-0767) bromophenol blue, 0 .2% xylene cyanole FF
Tris base 78.8 mg
50% Glycerol 5 ml
EDTA 14.6 mg
1.0% Bromophenol blue 2.0 ml
Xylene cyanole FF 20.0 mg
diH
2
O to 10 ml
Mini-PROTEAN Precast Gels