Chapter 2: setup and basic operation, 1 workflow overview, Setup and basic operation – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual
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Setup and Basic
Operation
2
2.1 Workflow Overview
Prepare Buffers
Prepare sample and running buffers
Prepare and Load Samples
Dilute in sample buffer
Prepare Gels and
Assemble Electrophoresis Cell
Analyze the Separation
(Chapter 10)
Perform Electrophoresis
SDS-PAGE (Chapter 3)
Native PAGE (Chapter 4)
Peptide Analysis (Chapter 6)
Nondenaturing Nucleic Acid PAGE (Chapter 7)
Denaturing Nucleic Acid PAGE (Chapter 8)
2-D Electrophoresis (Chapter 9)
Blot the Gels (Optional)
(Chapter 11)
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