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Chapter 2: setup and basic operation, 1 workflow overview, Setup and basic operation – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual

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Setup and Basic
Operation

2

2.1 Workflow Overview

Prepare Buffers

Prepare sample and running buffers

Prepare and Load Samples

Dilute in sample buffer

Prepare Gels and

Assemble Electrophoresis Cell

Analyze the Separation

(Chapter 10)

Perform Electrophoresis

SDS-PAGE (Chapter 3)

Native PAGE (Chapter 4)

Peptide Analysis (Chapter 6)

Nondenaturing Nucleic Acid PAGE (Chapter 7)

Denaturing Nucleic Acid PAGE (Chapter 8)

2-D Electrophoresis (Chapter 9)

Blot the Gels (Optional)

(Chapter 11)