Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 8)

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1.2 Gel Formulations
Mini-PROTEAN precast gels are composed of polyacrylamide with a bisacrylamide crosslinker, and they
are available in a range of formulations (Table 1.1) and in a selection of single percentages and gradients.
Table 1.1. Mini-PROTEAN precast gel formulations.
Application Gel Formulation Sample Buffer Running Buffer
SDS-PAGE Mini-PROTEAN TGX ™ Laemmli Tris/glycine/SDS
Mini-PROTEAN TGX Stain-Free
Native PAGE Mini-PROTEAN TGX Native Tris/glycine
Mini-PROTEAN TGX Stain-Free
Peptide analysis Mini-PROTEAN Tris-Tricine Tricine Tris/Tricine/SDS
dsDNA separation Mini-PROTEAN TBE Nucleic acid Tris/boric acid/EDTA (TBE)
ssDNA and RNA Mini-PROTEAN TBE-urea TBE-urea TBE
separation
1.3 Comb Configurations
Comb Type Well Volume
10-well 50 μl
10-well 30 μl
12-well 20 μl
15-well 15 μl
8 + 1 well* 30 μl
IPG/prep 7 cm ReadyStrip ™ IPG strip (450 μl)
1.4 Specifications
Gel material Polyacrylamide
Gel dimensions 7.2 x 8.6 cm
Gel thickness 1.0 mm
Resolving gel height 6.2 cm (5.6 cm for 50 μl well)
Cassette dimensions 8.5 x 10 cm
Cassette material Styrene copolymer
Comb material Polycarbonate
Running buffer 750 ml for 1–2 gels, 1,000 ml for 3–4 gels (Mini-PROTEAN Tetra cell)
325 ml for 1–2 gels (Mini-PROTEAN II or Mini-PROTEAN 3 cell)
* Multichannel pipet compatible.
Mini-PROTEAN Precast Gels