Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 32)

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Foam pad
Membrane
Gel
Filter paper
Filter paper
Foam pad
Assembly of the Mini-Trans-Blot cassette .
11.2.3 Transfer Using the Trans-Blot ® Turbo ™ System
1. Open the transfer pack and assemble the components on the cassette in the order shown. For best
results, use the roller to remove any air trapped between the layers. If using a single mini or midi
sandwich, place the sandwich in the middle of the cassette bottom. With two mini gels, place the
sandwiches on a midi stack with the foot of each gel facing the center of the stack.
2. Place the lid on the cassette and lock the lid in place by turning the knob clockwise, using the
symbols on the lid as a guide. Slide the cassette into the appropriate bay of the Trans-Blot Turbo cell.
Each cassette and bay can hold up to two mini gels or one midi gel (Table 11.1).
3. Start the transfer. With the cassette in the cell, press TURBO and select the gel type. Press A:RUN
or B:RUN to begin the transfer. Press LIST to select a Bio-Rad optimized protocol (Table 11.2) or a
user-defined protocol. Press NEW to create and run a new protocol.
4. When transfer completes, RUN COMPLETE appears. Pull the cassette straight out of the slot and
unlock the lid. Disassemble the blotting sandwich.
Refer to the Trans-Blot Turbo Instruction Manual (bulletin 10020688) for complete instructions.
Top ion reservoir stack
Gel
Cassette top
(–) electrode (cathode)
Membrane
Cassette bottom
(+) electrode (anode)
Bottom ion reservoir
stack
Assembly of the gel blot sandwich with the Trans-Blot Turbo system .
Mini-PROTEAN Precast Gels