Leica Biosystems RM2265 - Manual (Page 76)

74 Instructions for Use, V 1.8 RevC – 07/2013
7. Troubleshooting
Problem Possible cause Corrective action
• The blade is dull.
• The pressure plate is damaged
or incorrectly adjusted.
• Clearance angle of the knife/
blade too small.
• When using cassettes, par-
ticularly thin-walled cassettes,
from a company other than
Leica or Surgipath, the cassette
may become deformed or other
clamping problems may arise.
If while trying to clamp the cas-
sette you realize that it is not
securely clamped into place,
an alternative tensioning clamp
must be used.
• Laterally displace the knife
holder or insert a new blade.
• Insert a new pressure plate or
use a new knife holder.
• Methodically experiment with
larger clearance angle settings
until you have found the optimum
angle.
2. Section compression
The sections are very compressed,
show folds or are squeezed
together.
• The blade is dull.
• The specimen is too warm.
• The sectioning speed is too
fast.
• Use another area of the blade or
a new blade.
• Cool the specimen before sec-
tioning.
• Reduce the sectioning speed.
3. "Stripes" in sections
For knife holder E • There is an accumulation of par-
affin on the rear pressure plate
of the knife holder.
• Remove paraffin from this area
on a regular basis.