Leica Biosystems RM2265 - Manual (Page 66)

64 Instructions for Use, V 1.8 RevC – 07/2013
6. Optional Accessories
The freezer pack consists of the freezer plate (98)
and the insulation jacket (99). It is for cooling (or
keeping cold) paraffin specimens. Up to 35 stan-
dard cassettes can be cooled at the same time.
The specimens require approx. 20 min. for being
cooled from room temperature (approx. 20 °C) to
"sectioning temperature".
The cooling performance depends on the ambi-
ent temperature and the volume of the speci-
mens being cooled.
The insulation jacket can be placed on the hood
of the microtome in the same manner as the tray,
or it can be placed next to the instrument.
• Remove the freezer plate from the insulation
jacket and place it in a deep freezer, ideally
overnight (but for at least six hours) at approx.
-23 °C.
• Reassemble the freezer plate and insulation
jacket and place them on the bench or the
instrument.
6.7 Freezer pack
Fig. 57
98
99
98
6.8 LN22
Fig. 58 - LN22 (on Leica RM2265)
The Leica LN22 liquid nitrogen freezing attach-
ment has been developed to switch back and
forth between sectioning at room temperature
and frozen section applications quickly and
without problems.
Instrument characteristics:
Cryogenic chamber accessible from above -
cooled with liquid nitrogen - for creating frozen
sections in a condensate and ice-free chamber
at temperatures up to -150 °C. Backlighting, knife
holder etc. . .