Leica Biosystems CM1860/CM1860 UV - Manual (Page 53)

53 Leica CM1860/CM1860UV – Cryostat
7.5.3 Manual defrosting of the quick freeze shelf
The quick freeze shelf may become hot during defrosting! There-
fore, do not touch it!
In case of heavy frost formation on the quick freeze shelf, which can occur
particularly after the spray disinfection, a defrost can be activated manually
( see point 6.2.5 ). which can be terminated when required.
7.6 Terminating work
7.6.1 Terminating daily work
• Lock the handwheel.
• Take the knife out of the knife holder and put it back into the knife box in
the cryochamber.
• Remove frozen section waste with a cold brush.
• Empty the section waste tray.
• Clean the storage shelves and brush shelf.
• Remove all tools and supplies that would prevent the UV light from reach-
ing the chamber walls and shelves.
To clean and disinfect the interior of the instrument, use only alco-
hol-based disinfectants. On the exterior, common detergents and
alcohol-based disinfectants can be used.
All components removed from the cold environment will collect
condensation. Therefore, dry them thoroughly before placing them
back into the cryochamber.
• Remove all the specimens from the cryostat.
• Attach the cover for the freeze shelf (standard scope of delivery).
• Remove the plug and close the sliding window.
• Switch off the chamber illumination and start the UV disinfection.
• Lock control panel field 1 ( Fig. 14 ) using the key button.
• Do NOT switch off the instrument using the circuit breaker, as otherwise
no cooling takes place.
7. Daily Use of the Instrument
Be sure to place the corresponding cover on the quick freeze shelf
to prevent frost. Always cover the quick freeze shelf during breaks
and overnight.