Leica Biosystems CM1860/CM1860 UV - Manual (Page 35)

35 Leica CM1860/CM1860UV – Cryostat
7. Daily Use of the Instrument
7.1 Specimen freezing
• Select the sectioning temperature (chamber temperature) according to
the tissue type to be sectioned ( see "7.4 Temperature selection chart" ).
7.1.1 Quick freeze shelf
The cryochamber is equipped with a quick freeze shelf (5, Fig. 24) on which
up to 10 specimen stages with specimens can be attached.
The temperature of the quick freeze shelf is always lower than the cryo-
chamber temperature.
• Cut the specimen to size.
• If necessary, enable the Peltier element (4) - it
takes approx. 60 seconds until the maximum
cooling capacity is available.
• Apply a sufficient amount of cryocompound
to a specimen disc to orient and mount the
specimen.
• Place the specimen disc in one of the holes of
the quick freeze shelf and freeze the specimen
at a low temperature.
• Once the specimen is frozen, insert the speci-
men disc ( Fig. 25 ) with the specimen into the
specimen head ( Fig. 25 ) and start sectioning.
Fig. 24
4
1
2
5
3