Environmental Express AutoBlock II - Manual (Page 37)

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A custom method is built one step at a time by entering commands. Commands
include the following:
A) Inject
This command adds reagents. The inject range is 0.1-15mls in 0.1ml
increments.
To inject a reagent:
(1) Choose the appropriate reagent in the reagent drop down box.
(2) Enter the volume required via the keypad.
(3) Press Inject.
(4) To inject secondary reagents, repeat this process at the proper time in the
custom method.
Note: Purging the last reagent and prepping for the new reagent are
automatically taken into account by the ahb. program, and need not be
addressed in custom method development.
B) Heat To
This command determines the Set Point temperature, not sample temperature.
Range is 10°-125° C increments of 0.1°. For establishing accurate sample
temp, see section 5.3. Variable A or Variable B can be used in this field as
well. See section 5.2 for additional details. “A” is 95.0 sample temperature
without a reflux cap and “B” is 95.0 with a reflux cap. These are defaults.
Insure that they have not been modified in the maintenance mode.
C) Wait For
This command determines the amount of time the samples will be heated
during a step. Range is 1-999 minutes. The Wait For time does not start until
the Graphite temp reaches within 5 degrees of the Set Point temp. An
automatic command is sent for the elevator to lower the samples down into
the block, prior to the countdown.
D) Cool For
This command sets the amount of time the samples will be cooled. Range is
0.1-999 minutes. The speed of the fan automatically increases to it’s
maximum for efficient sample cooling.
Technical Note
When creating a custom method, samples must be cooled for a minimum of 20
minutes between heating and adding a reagent. Based on our tests, we
recommend you add 1-2 ml of DI Water prior to reagent additions. This helps
cool samples even further. HYDROGEN PEROXIDE can cause violent
reactions with some samples. A minimum of 35 minutes must be entered as a
cooling time, and you must add 2ml DI Water prior to the injection of H2O2.
7.7.1 Example of Custom Method Development