Environmental Express AutoBlock II - Manual (Page 20)

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5.2.1 Purging and Prepping
In the Manual mode, injecting reagents does not include purging the previous
reagent. Further, it does not include prepping with the new reagent, both of
which are important steps. Following are the steps for purging and prepping.
A) Purging
Purging is the process of flushing the lines of the previous reagent with DI
water.
Note: Always Purge with DI Water only!
1) Move the dispensing arm to a drain station
2) Select Reagent; H2O (Port #1)
3) Enter 3 in the Enter ml box via the keypad function
(see paragraph 4.1.2)
4) Press Inject
B) Prepping
Prepping is the process of removing the DI water from the lines with the
reagent to be injected into the samples.
1) Move the reagent dispensing arm to a drain station
2) Select reagent/port #
3) Enter 2 in the Enter ml box via the keypad function
(see paragraph 4.1.2)
4) Press Inject
Following purging and prepping, the AutoBlock is now ready to inject the
prepped reagent into the samples.
Note: In the Method mode, purging and prepping are automated features, and do
not need to be taken into account when designing a new method.
5.2.2 Reagent Injection
Note 1: For safety and cooling purposes, fully load the AutoBlock with 54 cups.
Note 2: Prior to adding reagents to cups, follow Purge and Prep procedures in
section 5.2.1.
A) Using left/right motion control, move the injection arm to the desired column
of samples.
B) Double-check that the correct Port #/reagent has been Prepped prior to
injection.
C) Enter the desired volume in the Enter ml box via the keypad function.
Reagent addition occurs in multiples of six. The Enter ml volume entered will
apply to each cup in each column.