Eppendorf 5430 R Centrifuge - Manual (Page 33)

Operation
Centrifuge 5430/5430 R
English (EN)
33
5.4.5 Loading the rotor
5.4.5.1 Fixed-angle rotors
To load the rotor, proceed as follows:
1. Check the maximum load (adapter, tube and contents) per rotor bore.
The information about this can be found on the rotor and in this operating manual (see Rotors on p. 12).
2. Load rotors and adapters only with the tubes intended for them.
3. Insert tubes opposite each other in pairs into the rotor bores. To ensure symmetric loading, tubes that
are arranged opposite each other must be of the same type and contain the same filling quantity.
To minimize weight differences between filled sample tubes, we recommend taring with a scale. This
will reduce wear on the drive and reduce running noise.
4. Attach and tighten rotor lid.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
 Load rotors symmetrically with identical tubes or plates and buckets.
 Only load adapters with suitable tubes or plates.
 Always use tubes or plates of the same type (weight, material/density and volume).
 Check that loading is symmetrical by balancing the adapters and tubes or plates used with
scales.
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone. Check the loading, balance the tubes and re-start the
centrifugation.
CAUTION! Risk from damaged or overloaded tubes.
 When loading the rotor, observe the safety precautions on dangers as a result of
overloaded or damaged tubes (see Warnings for intended use on p. 20).
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone.
 Check the load, balance the tubes and restart the run.
Rotor lid
• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. This is
clearly shown by the identical rotor name labeling on the rotor and on the rotor lid.
• To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (label: red ring) and the
corresponding aerosol-tight rotor lid (label: aerosol-tight and red lid screw) must be used.