Eppendorf 5430 R Centrifuge - Manual (Page 32)

Operation
Centrifuge 5430/5430 R
English (EN)
32
5.4 Preparing for centrifugation
5.4.1 Switching on the centrifuge
1. Switch on the centrifuge using the mains power switch or the standby key.
Only 5430: After switching on at the mains power switch, the centrifuge lid opens automatically.
2. Open the closed centrifuge lid by pressing the open key.
The parameter settings of the last run are displayed.
5.4.2 Inserting the rotor
1. Fit the rotor vertically on the motor shaft.
2. Insert the supplied rotor key into the rotor nut.
Rotor FA-45-24-11-HS: Use the special rotor key.
3. Turn rotor key clockwise until the rotor nut is firmly tightened.
5.4.3 Automatic rotor detection
The centrifuge has automatic rotor detection. It detects a newly inserted rotor during centrifugation and
displays its name for approx. 2 seconds. The set g-force (rcf)/speed (rpm) is automatically limited to the
maximum permissible value of the rotor, if necessary.
 Always check the set g-force (rcf)/speed (rpm) after a rotor change and adjust it if necessary.
5.4.4 Manual rotor detection
 In order to trigger the rotor detection manually, turn the rotor counterclockwise by hand.
• The name of the rotor appears in the display.
• The g-force (rcf)/speed (rpm) automatically is limited to the maximum value of the rotor.
 Swing-bucket rotors: remove the buckets before inserting and/or removing the rotor. Use
both hands to pick up the rotor cross.
 Rotor F-35-6-30: only use the rotor removal tool supplied to insert or remove the rotor.
If you start centrifuging immediately after a rotor change, the centrifuge has not carried out
an automatic rotor detection yet. The speed set for the previous rotor may exceed the
maximum permitted speed for the new rotor. In this case the centrifuge stops after the
automatic rotor detection and displays the error message
Note C . The new maximum
permitted speed appears in the display. You can then restart the centrifuging with this setting
or adjust the speed as necessary.
CAUTION! Risk of injury when turning the rotor manually.
 When turning a swing-bucket rotor, pay special attention to ensure that your fingers do not
get jammed or get caught on the swinging buckets.