Eppendorf BioSpectrometer basic - Manual (Page 97)

97
Evaluation procedure
Eppendorf BioSpectrometer ® basic
English (EN)
12.7 Dual wavelength
For methods of the Dual Wavelength group absorbances that were measured at two wavelengths can be
calculated with each other before the calculated absorbance is evaluated further with the factor or
standard.
To determine the calculated absorbance a division or subtraction evaluation can be defined in the
parameters:
A
1
, A
2
= measured absorbance.
a, b, c, d = factors that are entered in the parameters. Negative figures may also be entered.
d c
A b
A a
A
calc
u
u
u
2
1
> @
d c A b A a A
calc
u u u
2 1