Eppendorf BioSpectrometer basic - Manual (Page 96)

Evaluation procedure
Eppendorf BioSpectrometer
® basic
English (EN)
96
12.6.2 Calculation of the FOI
As a value for the ratio of dye molecules to the number of nucleotides in the nucleic acid the frequency of
incorporation (FOI) is calculated and displayed for the dye methods. The calculation can be selected for two
different result units:
MOLECULE dye/kb unit
pmole/μg DNA (or RNA) unit
A
YYY
= absorbance of the dye.
A
XXX
= absorbance of the nucleic acid.
MM
nt
= average molar mass of the nucleotides: 330 g/mol.
F
NA
= factor for calculating the nucleic acid
ε
dye
= absorbance coefficient for the dye, unit: cm -1 M -1
12.6.3 Conversion to amounts of dye
The quantity (dimensions) of dye in the entire sample volume is calculated in the process results/More
calculations method step.
M = calculated total amount (mass) of dye in the sample tube. Unit: pmol.
C = dye concentration calculated from the measurement. Unit: pmol/μL.
V
S, total
= total volume of the sample in the sample tube; entered by the user under More calculations. Unit:
μL.
NA XXX
nt
Dye
YYY
F A
MM A
FOI
u
u
u
6 10
H
NA XXX Dye
YYY
F A
A
FOI
u
u
9 10
H
total P
V C M
,
u