Eppendorf BioSpectrometer basic - Manual (Page 63)

63
Functions
Eppendorf BioSpectrometer ® basic
English (EN)
Tab. 7-2: Parameter in General Method Parameter
Parameter Explanation
Proteins These parameters are loaded into the method parameters when a protein
is selected during the programming of a method in the
Dye labels and
Proteins direct UV groups.
• Protein name
• Factor
• A
0.1%
• Ext.coeff.
• Molecular mass
In order to define a factor for calculating the concentration on the basis of
the absorbance, enter the following data in addition to the name and
wavelength:
Factor
or A
0.1%
or absorbance coefficient and molar mass.
Nucleic acids These parameters are loaded into the method parameters when a nucleic
acid is selected during the programming of a method in the
Dye labels
group.
• NA name
• Factor
• Double-stranded
The factor is used to calculate the concentration on the basis of the
absorbance.
The double-stranded parameter affects the calculation of the molar
nucleic acid concentration. (see
Conversion to molar concentrations and
nucleic acid quantities on p. 94)
Dyes These parameters are loaded into the method parameters when a dye is
selected during the programming of a method in the
Dye labels group.
• Dye name
• Wavelength
• Ext.coeff.
• Factor
• Corr. A260
• Corr. A280
In order to define a factor for calculating the concentration on the basis of
the absorbance, enter the following data in addition to the name:
Factor
or absorbance coefficient.
The correction factors for the absorbance values at 260 or 280 nm are
used when the correction function in the method parameters is active. For
more details, refer to the chapter on evaluation (see
Correction A
260
and
correction A
280
on p. 93).
Units You can select a unit from all available units when programming method
parameters.
• Unit Entering a unit that has not yet been programmed for the concentration
result.
• Specifications for proteins which are not preset at the factory can be determined in the
expasy database: http://www.expasy.org/tools/protparam.html.
• A table with A
1%
values for many proteins can also be found in: C.N.Pace et al., Protein
Science (1995), 4: 2411–2423 (Table 5). The A
1%
values must be multiplied by 0.1 to
return the required A
0.1%
values.