Eppendorf BioSpectrometer basic - Manual (Page 53)

53
Methods
Eppendorf BioSpectrometer ® basic
English (EN)
More calculations
Press the [More calc.] softkey.
Nucleic acids method group:
• After the molar mass has been entered (in base/
base pairs or in kDa): Convert the concentration
result to the molar concentration.
• After the sample volume has been entered:
Calculate the total amount in the sample.
Dye labels method group:
Nucleic acid:
• After the molar mass has been entered (in base/
base pairs or in kDa): Convert the concentration
result to the molar concentration.
• After the sample volume has been entered:
Calculate the total amount in the sample.
Dye:
• After entering the volume of the sample:
Calculate the total amount in the sample.
• For dsDNA the calculation of the molar concentration is based on the assumption of a
double-stranded nucleic acid. For the ssDNA, RNA and Oligo methods, a single-stranded
nucleic acid is assumed.
• For methods which have been reprogrammed via in the Routine main
group, Nucleic acids method group, always double-stranded nucleic acids are assumed
for calculating the molar concentration.