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Eppendorf BioSpectrometer basic User Manual

Page 36

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Methods
Eppendorf BioSpectrometer

®

basic

English (EN)

36

Unit

Selection:
mg/mL | μg/mL | ng/
mL | pg/mL | μg/μL |
mg/dL | μmol/mL |
nmol/mL | pmol/mL |
pmol/μL | U | U/mL |
U/L | % | Abs | A/min
In addition, further
units are freely
programmable in the
General Method
Parameters/Units
function. Max. 7 digits.

Unit for the concentration result.
In the preprogrammed methods of the

Routine group, the

selection is restricted to units that are useful for these methods.

Formula type

Selection:
division | subtraction

Only for the

Dual wavelength method group.

Formula type for calculating the absorbances at the two
measurement wavelengths prior to evaluation with factor or
standard.

Formula:

a

Value input:
Value for

a in the

evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for

a in the formulas: [(a*A1) / (b*A2)] * c + d and [(a*A1) -

(b*A2)] * c + d.

Formula:

b

Value input:
Value for

b in the

evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for

b in the formulas: [(a*A1) / (b*A2)] * c + d and [(a*A1) -

(b*A2)] * c + d.

Formula:

c

Value input:
Value for

c in the

evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for

c in the formulas: [(a*A1) / (b*A2)] * c + d and [(a*A1) -

(b*A2)] * c + d.

Formula:

d

Value input:
Value for

d in the

evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for

d in the formulas: [(a*A1) / (b*A2)] * c + d and [(a*A1) -

(b*A2)] * c + d.

Calculation

Selection:
factor | standard

Evaluation procedure for the calculation of the sample
concentration from the measured absorbance.

Parameter

Entry

Explanation