Eppendorf BioSpectrometer basic - Manual (Page 81)

81
Troubleshooting
Eppendorf BioSpectrometer ® basic
English (EN)
• Calculation not
possible
because of
division by zero.
Absorbance
result is zero.
• Calculation
error. Division
by zero.
• The evaluation required dividing by
an absorbance result with the value of
"zero". This is not mathematically
permissible.
Examples: Calculation of a factor at
one-point calibration; calculation of a
260/280 ratio with nucleic acid
measurements.
 Check the reagents and samples used
and repeat the measurement.
Calculation not
possible because of
division by zero.
Absorbance result
or parameter
formula
b is zero.
• An absorbance result was divided by
a "zero" value during the evaluation
of a
Division type method (Dual
wavelength method group). This is
not mathematically permissible.
 Check the reagents and samples used
and repeat the measurement.
 Do not enter "zero" as a value for the
Formula
b parameter.
Problem Cause Solution