Eppendorf BioSpectrometer basic - Manual (Page 54)

Methods
Eppendorf BioSpectrometer
® basic
English (EN)
54
Peak detection
Press the [Peaks] softkey. For the peak detection you can alternate between two criteria:
• λ grid: Evaluation grid on the wavelength scale for the peak detection (e.g., 10 nm).
10 nm example: The spectra section from -5 nm to +5 nm is evaluated in relation to the peak to be
detected.
• Min. Δ Abs: Minimum difference between the peak to be detected and the lowest absorbance in the
evaluation grid. No absorbance value in the grid may be higher than the value of the peak at any given
time (e.g.: 0.5).
Examples:
λ grid: 100 nm, min. Δ Abs: 0.050:
The peak is not detected because the λ grid is too
large: The absorbance values on the left edge of the
grid are higher than the absorbance of the peak.
λ grid: 20 nm, min. Δ Abs: 0.200:
The peak is not detected because the predetermined
value for
min. Δ abs is too high. The difference of the
absorbance of the peak and the lowest absorbance in
the grid is less than 0.2 A.
λ grid: 20 nm, min. Δ Abs: 0.050:
The peak is detected.