Eppendorf BioSpectrometer basic - Manual (Page 90)

Evaluation procedure
Eppendorf BioSpectrometer
® basic
English (EN)
90
12.1.3 Cuvette correction
All absorbance values which are used for result calculation are standardized to the cuvette layer thickness
of 10
mm. If a cuvette with a different path length is used, this path length must be defined in the cuvette
parameter. In this case, the measured absorbances are corrected to match measuring results with a cuvette
layer thickness of 10
mm before converting them to sample results.
This correction is applied to:
• Methods with evaluation by factor.
• Methods of the Absorbance group, for which only absorbance values are output
The correction is not applied to:
• Methods with evaluation by standards, as we presume that standards and samples are measured in
cuvettes of the same layer thickness.
• Calculations with division: Division method (Dual wavelength method group) and calculation of ratios
such as A
260
/A
280
(for nucleic acid measurements).
A
XXX, corrCuv
= calculated corrected absorbance at a wavelength of XXX nm.
A
XXX
= measured absorbance at a wavelength of XXX nm.
Cuv = path length of the cuvette.
Cuv
A A
XXX corrCuv XXX
10
,
u