Jenway 7315 Manual Italian - Manual (Page 42)

Once this measurement has been performed the standard concentration samples can be measured.
Remove the cuvette containing the blank solution from the sample chamber and insert the cuvette
containing the first standardised solution to be measured.
Close the instrument lid and press the key adjacent to
the
tick icon to take a reading of the standard. The
concentration along with the photometric value will
then be displayed. This standard can be re-measured by
pressing the key adjacent to the
back icon.
To measure the next standard remove the current standard from the sample chamber and insert the
cuvette containing the second standardised solution. Press the key adjacent to the
tick icon to take a
reading of the standard. Repeat this procedure until all the standards (up to a maximum of six) have been
measured. Once all the standards have been measured the instrument returns to the standard curve
screen and the new curve can be viewed. The photometric values for all of the newly added standards
can be viewed in the quantitation table.
To abort creation of the new standard curve before all the standards have been measured press the key
adjacent to the
cross icon at any time and the instrument will return to the previous standard curve.
Once the curve has been created press the key adjacent to the tick icon and the screen will return to
the expanded operating menu where unknown samples can be measured. When this operating mode is
exited the current standard curve will be saved until it is replaced by a new curve.
The automatic 8 cell turret can be used to assist creating a new curve. Refer to section 11.3.1.1 for more
details.
8.3 CALiBRAtion
The zero calibration must be performed at the same
wavelength as the standards were measured at and the
unknown samples will be measured at. Insert a cuvette
containing the blank solution into the sample chamber
and close the instrument lid. Press the key below the
calibrate to zero absorbance icon. The instrument will
calibrate to zero absorbance.
Once the calibration is complete the measure sample icon appears and the sample can be measured.
If the wavelength is adjusted before a sample is measured the measure sample icon will disappear and
the instrument must be calibrated again at the new wavelength.
8.4 SAMPLe MeASUReMent
Once a calibration has been performed the measure
sample icon appears. A standard curve should either be
created or loaded from a previously saved method before
carrying out a measurement of an unknown sample.
Place the cuvette containing the sample in the sample
chamber and close the instrument lid.
Press the key below the measure sample icon and the instrument will take a reading and the
concentration and photometric values will be displayed on the screen. To view the position of this sample
on the standard curve open the standard curve screen.
42
0.000 ABS
1: 0.10 ppm
0.000
0.000
400
09:02
ppm
ABS
nm
0.986
0.000
400
09:02
ppm
ABS
nm