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PASCO PS-2108 Dissolved Oxygen Sensor User Manual

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Model No. PS-2108

Dissolved Oxygen Sensor

Table of Contents

Equipment List .......................................................... 1
Introduction ............................................................. 1
DO Sensor Setup and Calibration...................................... 3

Prepare the Sensor for First Use .................................................................................................... 3
Set up the Dissolved Oxygen Sensor with DataStudio.................................................................. 3
Equilibrate the Probe in 100% Humidified Air ............................................................................. 4
Perform a Single-point Calibration (mg/l Dissolved O2) .............................................................. 4
Setup to Calibrate in Percent (%) Saturation ................................................................................. 4

General Sampling Procedure............................................ 6

Measurements in a Controlled Laboratory Setting ........................................................................ 6
Using the Dissolved Oxygen Sensor with other PASCO Sensors:................................................ 6

Maintenance ............................................................. 7

Changing the Electrolyte solution.................................................................................................. 7
Replacing the Membrane ............................................................................................................... 7
Replacing the O-ring...................................................................................................................... 8

Storage .................................................................. 9

Short-Term Storage (two weeks or less)........................................................................................ 9
Long-Term Storage (more than two weeks) .................................................................................. 9

Troubleshooting ......................................................... 9
Theory of Dissolved Oxygen: ......................................... 10

What is the chemical mechanism by which diatomic oxygen dissolves in water?...................... 10
Citations: ...................................................................................................................................... 14

Experiment 1:

Introduction to the Operation of the Dissolved O2 Sensor ........ 15

Purpose......................................................................................................................................... 15
Materials and Equipment Needed ................................................................................................ 15
Optional Equipment Suggested ................................................................................................... 15
Procedure ..................................................................................................................................... 15
Data Analysis/Questions.............................................................................................................. 16

Experiment 2:

Photosynthesis and Oxygen Generation with Aquatic Plants ........ 17

Purpose......................................................................................................................................... 17
Materials and Equipment Needed ................................................................................................ 17
Procedure ..................................................................................................................................... 18
During the lab time ...................................................................................................................... 18
Collecting Data ............................................................................................................................ 19
Sample Data ................................................................................................................................. 19
Analysis ....................................................................................................................................... 19