Sensitivity, Capacity, Optics – Luminex 100E Hardware User Manual User Manual
Page 16
Luminex 100E User Manual
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MAP Technology
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PN 89-00002-00-013 Rev. D
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Physical dimensions, excluding mounting brackets, connectors,
and tubing, are: 31.1 cm (12.25 inches) W x 50 cm (19.7 inches)
D x 29.2 cm (11.5 inches) H
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Weight: 21.8 kg (48.0 lbs.)
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Shipping and storage: The allowable shipping and storage
temperature and humidity ranges are 0°C to +50°C and up to
80% noncondensing
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Underwriters Laboratories, Inc. (UL
®
) installation category: UL
Installation Category II, as defined in Annex J of UL 61010A-1
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Pollution degree: UL Pollution Degree II, as defined in Section
3.7.3.2 of UL 61010A-1
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Classification of microspheres > 80%
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Misclassification of microspheres < 0.5%
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Internal sample carry over < 3%
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Soluble background fluorescence emission at 575 nm
automatically subtracted from median fluorescence intensity
(MFI) value
Sensitivity
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Detect 1000 fluorochromes phycoerythrin (PE) per microsphere
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Reporter channel dynamic range: 3.5 decades of detection
Capacity
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Sample core: 15 - 20 µm core at 1 µL/sec sample injection rate
Optics
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Reporter laser: 532 nm, nominal output 15-16.5 mW, maximum
of 500 mW, frequency-doubled diode; mode of operation,
continuous wave (CW)
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Classification laser: 635 nm, 9.1 mW ± 6%, maximum output
25 mW, diode; mode of operation, continuous wave (CW)
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Reporter detector: Photomultiplier tube, detection bandwidth of
565 - 585 nm
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Classification detector: Avalanche photo diode (APD) with
temperature compensation
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Doublet discrimination detector: Avalanche photo diode (APD)
with temperature compensation