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Sensitivity, Capacity, Optics – Luminex 100E Hardware User Manual User Manual

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Luminex 100E User Manual

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MAP Technology

3 - 4

PN 89-00002-00-013 Rev. D

Physical dimensions, excluding mounting brackets, connectors,
and tubing, are: 31.1 cm (12.25 inches) W x 50 cm (19.7 inches)
D x 29.2 cm (11.5 inches) H

Weight: 21.8 kg (48.0 lbs.)

Shipping and storage: The allowable shipping and storage
temperature and humidity ranges are 0°C to +50°C and up to
80% noncondensing

Underwriters Laboratories, Inc. (UL

®

) installation category: UL

Installation Category II, as defined in Annex J of UL 61010A-1

Pollution degree: UL Pollution Degree II, as defined in Section
3.7.3.2 of UL 61010A-1

Classification of microspheres > 80%

Misclassification of microspheres < 0.5%

Internal sample carry over < 3%

Soluble background fluorescence emission at 575 nm
automatically subtracted from median fluorescence intensity
(MFI) value

Sensitivity

Detect 1000 fluorochromes phycoerythrin (PE) per microsphere

Reporter channel dynamic range: 3.5 decades of detection

Capacity

Sample core: 15 - 20 µm core at 1 µL/sec sample injection rate

Optics

Reporter laser: 532 nm, nominal output 15-16.5 mW, maximum
of 500 mW, frequency-doubled diode; mode of operation,
continuous wave (CW)

Classification laser: 635 nm, 9.1 mW ± 6%, maximum output
25 mW, diode; mode of operation, continuous wave (CW)

Reporter detector: Photomultiplier tube, detection bandwidth of
565 - 585 nm

Classification detector: Avalanche photo diode (APD) with
temperature compensation

Doublet discrimination detector: Avalanche photo diode (APD)
with temperature compensation