Luminex 100E Hardware User Manual - Manual (Page 13)

PN 89-00002-00-013 Rev. D 3 - 1
Luminex 100E Analyzer Overview
Theory of Operation
The Luminex 100E analyzer was specifically designed to be a
component of a host system.
Luminex 100E analyzer technology is based on flow cell fluorometry
with Luminex-developed innovations. The fluidics and optics work
together to enable simultaneous analysis of up to 25 analytes in a
single test sample using the Luminex 25 beadmap.
There are two fluidics paths in the Luminex 100E analyzer. The first
path involves a syringe-driven mechanism that draws the sample into
the Luminex 100E analyzer for analysis. The host system drives the
second fluidics path at a controlled rate to supply sheath fluid to the
cuvette. The Luminex analyzer’s syringe-driven system transports a
specified volume of sample from a sample container to the cuvette.
The sample is injected into the cuvette at a steady rate for analysis.
Following analysis, the sample path is automatically purged with
sheath buffer by the second fluidics path. This process removes
residual sample within the tubing, valves, and probe.
Sheath fluid is the delivery medium of the sample to the optics
component. The analysis sample is acquired using the host system’s
sample aspiration interface and is injected into the base of the
cuvette. The sample passes through with sheath fluid at a controlled
rate in a narrow sample core to ensure that each microsphere is
illuminated individually. The sample injection rate is such that the
microspheres are introduced to the optics path as a series of single
events.
The optics assembly consists of two lasers. One laser excites the dye
mixture inside the microspheres, and the second laser excites the dye
bound to the surface of the microspheres. Avalanche photo
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