Olympus IX71 - Manual (Page 46)

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1. Rotate the condenser turret to engage the suitable optical element for
the objective in use in the light path.
2. Engage the objective to be used in the light path.
3. Place the specimen on the stage and bring the specimen into focus by
moving the objective up or down.
4. Adjust the field iris diaphragm so that its image circumscribes the field of
view.
5. Adjust the aperture iris diaphragm to enhance the contrast.
6. Move the prism movement knob of the DIC slider to select the interference
color that can provide the optimum contrast in accordance with the
specimen.
U-DICT:
The background interference color is continuously variable
from the gray sensitive color to purple sensitive color.
U-DICTS:
U-DICTHC:
U-DICTHR:
}When in sensitive color observation using the U-UCD8, engage the U-
UCDTP530 1- plate (sensitive color plate) in the light path.
· Setting the background color to dark enables an observation like darkfield
observation.
· Setting the background color to gray provides observation with high
contrast and 3D feeling with the gray sensitive color with which the
sensitivity is highest.
· Setting the background color to gray allows very small change in phase
to be observed as a change in color.
· There is a directional characteristic with the detection sensitivity because of
the configuration of the DIC prism. As a result, the contrast may sometimes
be improved by rotating the specimen on the stage.
# For simultaneous observation with reflected fluorescence observation,
refer to the instruction manual entitled “Reflected Fluorescence
System”.
6 Observation Method
The back ground interference color is continuously variable
from black to light gray.