Olympus IX71 - Manual (Page 29)

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IX71/IX51
Fig. 36
4-6 Illumination Column IX2-ILL30
}This illumination column is provided with a condenser as standard. The applicable objectives range from 4X to 40X
objectives. The field iris diaphragm is fixed.
It is not equipped with a condenser holder so no condenser can be attached to this illumination column.
1 Using the Aperture Iris Diaphragm (Figs. 36 & 37)
}The aperture iris diaphragm adjusts the numerical aperture of the
illumination system in brightfield observation. It determines the focal
depth, contrast and resolution according to purpose.
· To check the aperture iris diaphragm, remove an eyepiece (and attach
the U-CT30 if this is available) and look into the eyepiece sleeve. The
aperture iris diaphragm can be seen as shown in Fig. 36. Its aperture can
be adjusted by rotating the aperture iris diaphragm lever @.
}When the U-BI90CT binocular tube is used, the aperture iris diaphragm
can be viewed by setting the turret to position “CT”.
· In general, with dyed specimens, optimum observation is possible by
controlling the aperture iris diaphragm to between 70% and 80% of the
aperture number of the objective. With culture specimens that are not
dyed, adjust the aperture iris diaphragm lever by rotating it in the
direction of as required.
2 Removing the Condenser Lens (Fig. 37)
}The working distance can be increased by removing the condenser ²
by rotating its bottom part in the clockwise direction when it is seen
from above. However, the illumination becomes inappropriate. Use this
method as a simplified method when using only large culture vessels.
Fig. 37
@
²
Aperture iris
diaphragm image
70-80%
30-20%
Objective’s
exit pupil